EPINEPHRINE SUPPRESSES SECRE TION ORVLDLASSOCIATED TRIACYLGLYCEROLAND INCREASES TRIACYLGLYCEROL AND PHOSPHOL IPID CON TEN TS IN ISOL ATED RAT HEPATOCY TES
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Abstract:
The effect of epinephrine on triacylglycerol secretion was investigated in isolated rat hepatocytes. The effect appeared at concentrations of more than 1 µM and reached a plateau at 10 µM. Epinephrine concentration for half of the maximal bioeffect (EC50) was about 1 µM. Epineplrrine at a concentration of 10 µM suppressed triacylglycerol secretion by 33% and increased its cellular content by approximately 18%. The total triacylglycerol content of the system (sum of the cell and the incubation medium) was constant at all concentrations of epinephrine. Time course experiments for triacylglycerol secretion exhibited relatively similar results on the basis of lipid analysis with or without lipid extractions. On the former basis triacylglycerol secretion versus time followed a linear relationship with a slope of 1.47 ± 0.11 µmole TG/3h/ g wet liver. Time course curves of cellular lipids revealed that cellular triacylglycerol and phospholipid contents in the presence of epinephrine were higher than the control at all time points, and the difference was constant during time. Furthermore, in the presence of glucose (20 mM) and oleate (0.25 mM), intracellular triacylglycerol content increased markedly (≥45%) whereas cellular phospholipid content remained constant. It is proposed that epinephrine exerts an inhibitory effect on VLDL secretion probably through blocking in the secretory pathway.
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Journal title
volume 14 issue 1
pages 61- 68
publication date 2000-05
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